test organisms enterococcus faecalis  (ATCC)

Journal: Antibiotics

Article Title: Evaluation of the Inactivation of Microorganisms by a Blue Laser (445 nm)—An In Vitro Study

doi: 10.3390/antibiotics15020137

Figure Lengend Snippet: A box plot of the log 10 -transformed colony-forming unit (CFU) values of Enterococcus faecalis , Streptococcus mutans , and Candida albicans in untreated samples (negative controls), laser-treated samples (test groups 4 mm/s, 2 mm/s, and 1 mm/s), and positive controls treated with chlorhexidine digluconate (CHX). Data source .

Article Snippet: The test organisms Enterococcus faecalis (ATCC ® 29212TM) , Streptococcus mutans (DSM 20523/ATCC ® 25175TM), and Candida albicans (SC5314/ATCC MYA-2876D-5TM) were used for the main experiments: two Gram positive bacteria and one fungus as representatives of important dental pathogens.

Techniques: Transformation Assay

Journal: Antibiotics

Article Title: Evaluation of the Inactivation of Microorganisms by a Blue Laser (445 nm)—An In Vitro Study

doi: 10.3390/antibiotics15020137

Figure Lengend Snippet: Testing of the carrier solutions: phosphate buffered saline (PBS), deionised H 2 O, potassium phosphate buffer (KPO 4 ), sodium potassium phosphate buffer (NaKPO 4 ) and Tris-buffered saline with hydrochloric acid (Tris-HCl) by applying the microorganisms to the ceramic test specimens, vital staining using MTT, and subsequent microscopic evaluation of the microbial converted blue formazan. Tris-HCl reduced the viability slightly for Enterococcus faecalis and strongly for Streptococcus mutans . Sodium potassium phosphate buffer was not suitable for any of the test organisms. PBS was also not optimal for this test setup, with clearly recognisable salt crystals forming. Stable viability results were shown with the H 2 O (deionised) solution for Candida albicans and Enterococcus faecalis and with the potassium phosphate buffer solution for Streptococcus mutans . Figure source .

Article Snippet: The test organisms Enterococcus faecalis (ATCC ® 29212TM) , Streptococcus mutans (DSM 20523/ATCC ® 25175TM), and Candida albicans (SC5314/ATCC MYA-2876D-5TM) were used for the main experiments: two Gram positive bacteria and one fungus as representatives of important dental pathogens.

Techniques: Saline, Staining

Journal: Journal of medicinal chemistry

Article Title: N-O Chemistry for Antibiotics: Discovery of N -Alkyl- N -(pyridin-2-yl)hydroxylamine Scaffolds as Selective Antibacterial Agents Using Nitroso Diels-Alder and Ene Chemistry

doi: 10.1021/jm200794r

Figure Lengend Snippet: Spectrum of antibacterial activity for compounds 7a , 9 , and 11a in the agar diffusion assay. a , b , c

Article Snippet: Test Organism M. luteus ATCC 10240 S. aureus SG511 E. faecalis ATCC 49532 Zone (mm) MIC 90 (μM) Zone (mm) MIC 90 (μM) Zone (mm) MIC 90 (μM) 7a 34 4.0 12 >128 16 * >128 7b 34 4.0 18 * ≥128 20 * >128 9 48 4.0 12 >128 15 * >128 11a 35 8.0 19 64 20 * >128 12 31 8.0 18 128 22 * >128 14 31 8.0 13 128 13 * >128 16 21 64 0 >128 13 * >128 18a 52 2.0 11 >128 22 * >128 18b 29 4.0 20 128 22 * >128 18c 25 16 10 >128 19 * >128 18d 40 2.0 18 128 21 * ≥128 18e 35 4.0 17 * >128 19 * >128 18f 38 2.0 12 >128 19 * >128 18g 27 16.0 0 >128 14 * >128 18h 35 4.0 13 >128 12 * >128 18i 15 128 0 >128 13 * >128 19a 33 4.0 15 128 18 * >128 19b 20 64 19 32 18 * >128 19c 30 16 15 * 128 16 * >128 30d 0 >128 0 >128 0 >128 31a 18 * ≥128 0 >128 0 >128 31b 13 * >128 0 >128 0 >128 31c 15 128 17 * >128 16 * >128 31d 24 64 18 128 20 * >128 31e 16 128 0 >128 15 * >128 31f 14 >128 0 >128 12 * >128 31g 12 * >128 13 >128 0 >128 31h 16 128 15 * >128 15 * >128 31i 17 128 14 * >128 15 * >128 32 0 >128 0 >128 0 >128 33 20 128 12 >128 11 * >128 34 0 >128 0 >128 0 >128 35 22 32 0 >128 0 >128 37 15 * ≥128 16 * >128 0 >128 39 0 >128 0 >128 0 >128 40a 31 2.0 18 * >128 19 * >128 cipro d 0 8.0 26 0.5 17 2.0 Open in a separate window a Exactly 50 μL of each compound solution (2.0 mM in 10:1 MeOH:DMSO) were added to 9 mm wells in agar media (MHII) inoculated with ~5×10 3 CFU/mL.

Techniques: Activity Assay, Diffusion-based Assay